Institut für Laboratoriumsmedizin, Klinische Chemie und Pathobiochemie 

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Publikationen

Hier finden Sie Publikationen der AG Blanchard.

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Publikationen

  • Blanchard V, Gadkari RA, Dighe RR, Leeflang BR, Gerwig GJ and Kamerling JP (2007) Characterisation of the N-linked oligosaccharides from human chorionic gonadotropin expressed in the methylotrophic yeast Pichia pastoris. Glycoconjugate J., 24, 33-47.
  • Blanchard V, Chevalier F, Imberty A, Leeflang BR, Basappa, Sugahara K, and Kamerling JP (2007) Conformational studies on five octasaccharides isolated from Chondroitin Sulfate using NMR spectroscopy and molecular modeling. Biochemistry, 46, 1167-1175.
  • Blanchard V*, Gadkari RA*, Roy S, Gerwig GJ, Leeflang BR, Dighe RR, Boelens R and Kamerling JP (2008b). High-level expression of biologically active glycoprotein hormones in Pichia pastoris strains GS115, and not X-33, for the production of biologically active N-glycosylated (15)N-labeled Glycoconj J, 25(3):245-57.
  • Blanchard V, Frank M, Leeflang BR, Boelens R, and Kamerling JP (2008) The structural basis of the difference in sensitivity for PNGase F in the de-N-glycosylation of the native bovine pancreatic ribonucleases B and BS. Biochemistry, 47(11):3435-46.
  • Wedepohl S, Kaup M, Riese SB, Berger M, Dernedde J, Tauber R, and Blanchard V (2010) N-Glycan Analysis of Recombinant L-Selectin Reveals Sulfated GalNAc and GalNAc-GalNAc Motifs. J Proteome Res, 9(7):3403-11.
  • von Horsten HH, Ogorek C, Blanchard V, Giese C, Demmler C, Winkler K, Kaup M, Berger M, Jordan I, and Sandig V (2010) Production of non-fucosylated antibodies by co-expression of heterologous GDP-6-deoxy-D-lyxo-4-hexulose reductase. Glycobiology, 20(12):1607-18.
  • Tansi FL , Blanchard V, Berger M, Tauber R, Reutter W, and Fan H (2010) Interaction of human dipeptidyl peptidase IV and human immunodeficiency virus type-1 transcription transactivator in Sf9 cells. Virology Journal,7:267.
  • Lieke T*, Gröbe D*, Blanchard V*, Grunow D, Tauber R, Zimmermann-Kordmann M, Jacobs T, and Reutter W (2011) Invasion of Trypanosoma cruzi into host cells is impaired by N-propionylmannosamine and other N-acylmannosamines. Glycoconjugate journal, 28(1):31-7.
  • Blanchard V*, Liu X*, Eigel S, Kaup M, Rieck S, Janciauskiene S, Sandig V, Marx U, Walden P, Tauber R, and Berger M (2011). N-glycosylation and biological activity of recombinant human Alpha1-Antitrypsin expressed in a novel human neuronal cell line. Biotechnology and Bioengineering, 108(9):2118-28.
  • Krentsis IM, Sela I, Eiges R, Blanchard V, Berger M, Becker Cohen M, and Mitrani-Rosenbaum S (2011) GNE is involved in the early development of skeletal and cardiac muscle. Plos One, 6(6):e21389.
  • Kaup M*, Saul VV*, Lusch A, Blanchard V, Tauber R and Berger M. Construction and analysis of a novel peptide tag containing an unnatural N-glycosylation site (2011) FEBS Letters, 585(14):2372-6.
  • Blanchard V, Kaup K, Eigel S, Rieck S, Sandig V, Marx U, Tauber R, and Berger, M. Quantitative MALDI-TOF-MS using stable-isotope labeling - Application to the analysis of N-glycans of recombinant alpha-1 antitrypsin produced using different culture parameters (2011) Journal of Carbohydrate Chemistry, 30:4-6, 320-333.
  • Frisch E, Kaup M, Egerer K, Weimann A, Tauber R, Berger M*, Blanchard V*. Profiling of Endo H-released serum N-glycans using CE-LIF and MALDI-TOF-MS - Application to rheumatoid arthritis (2011) Electrophoresis, 32(24):3510-5.
  • Reinke SO, Bayer M, Berger M, Blanchard V and Hinderlich S. Analysis of cell surface N-glycosylation of the human embryonic kidney 293T cell line (2011) Journal of Carbohydrate Chemistry, 30:4-6, 218-232.
  • Oefner CM, Winkler A, Hess C, Lorenz AK, Holecska V, Huxdorf M, Schommartz T, Petzold D, Bitterling J, Schoen AL, Stoehr AD, Vu Van D, Darcan Y, Blanchard V, Schmudde I, Laumonnier Y, Ströver HA, Hegazy AN, Eiglmeier S, Schoen CT, Mertes MMM, Loddenkemper C, Löhning M, König P, Jappe U, Petersen A, Luger E, Collin M, Köhl J, Hutloff A, Hamelmann E, Berger M, Wardemann H, Ehlers M (2012) Tolerance induction with T cell-dependent protein antigens induces regulatory sialylated IgGs. Journal of Allergy and Clinical Immunology, 129(6):1647-55.
  • Werner A, Horstkorte R, Glanz D, Biskup K, Blanchard V, Berger M, Bork K (2012) Glycoengineering the N-acyl side chain of sialic acid of human erythropoietin affects its resistance to sialidases. Biol Chem, 393(8): 777–783.
  • Reinke SO, Berger M, Hinderlich S* and Blanchard V* (2012) The analysis of N-glycans of cell membrane proteins from human hematopoietic cell lines reveals distinctions in their pattern. Biological Chemistry, 393(8): 731–747.
  • Karsten CM, Pandey MK, Figge J, Kilchenstein R, Taylor PR, Rosas M, McDonald JU, Orr SJ, Berger M, Petzold D, Blanchard V, Winkler A, Hess C, Reid DM, Majoul IV, Strait RT, Harris NL, Köhl G, Wex E, Ludwig R, Zillikens D, Nimmerjahn F, Finkelman FD, Brown GD, Ehlers M, Köhl J (2012) Anti-inflammatory activity of IgG1 mediated by Fc galactosylation and association of FcγRIIB and dectin-1. Nat Med, 18(9) : 1401-1406.
  • Frisch E, Schwedler C, Kaup M, Braicu EI, Gröne J, Lauscher JC, Sehouli J, Zimmermann M, Tauber R, Berger M, and Blanchard V (2013) Endo H de-N-glycosylation in a domestic microwave oven, application to biomarker discovery. Anal Biochem, 433: 65-69.
  • Hess C, Winkler A, Lorenz AK, Holecska V, Blanchard V, Eiglmeier S, Schoen CT, Bitterling J, Schoen AL, Stoehr AD, Petzold D, Schommartz T, Mertes MMM, Tiburzy B, Herrmann A, Köhl J, Manz M, Madaio MP, Berger M, Wardemann H and Ehlers M (2013) T cell-independent B cell activation induces immunosuppressive sialylated IgG antibodies. J Clin Inv., 123(9):3788-96.
  • Biskup K, Braicu EI, Sehouli J, Fotopoulou C, Tauber R, Berger M and Blanchard V (2013) Serum glycome profiling - a biomarker for diagnosis of ovarian cancer. J Prot Res, 12(9):4056-63.
  • Lusch A, Kaup M, Marx U, Tauber R, Blanchard V* and Berger M* (2013) Development and analysis of alpha 1-antitrypsin Neoglycoproteins - the impact of additional N-glycosylation sites on serum half-life. Mol Pharm, 10(7):2616-29.
  • Hamouda H*, Ullah M*, Berger M, Sittinger M, Tauber R, Ringe J and Blanchard V (2013) N-Glycosylation profile of undifferentiated and adipogenic differentiated human bone marrow mesenchymal stem cells - towards a next generation of markers for regenerative medicine. Stem Cells and Development, 22(23):3100-13.
  • Schwedler C, Kaup M, Petzold D, Hoppe B, Braicu EI, Sehouli J, Ehlers M, Berger M, Tauber R, Blanchard V (2014) Sialic acid methylation refines CE-LIF analyses of IgG N-glycans of ovarian cancer patients, Electrophoresis, 35(7):1025-31.
  • Biskup K, Braicu EI, Sehouli J, Tauber R and Blanchard V (2014) The serum glycome to discriminate between early-stage epithelial ovarian cancer and benign ovarian diseases. Dis Markers, 2014:238197.
  • O’Regan NL, Steinfelder S, Schwedler C, Rao GB, Srikantam A, Blanchard V and Hartmann S. Filariasis asymptomatically infected donors have lower levels of disialylated IgG compared to endemic normals. Parasite Immunol, 36(12):713-20.
  • Schwedler C, Kaup M, Weiz S, Hoppe M, Braicu EI, Sehouli J, Hoppe B, Tauber R, Berger M and Blanchard V (2014) Identification of 34 N-glycan isomers in human serum by Capillary Electrophoresis allows identifying increases of core-fucosylated structures in ovarian cancer patients. Anal Bioanal Chem, 406(28):7185-93.
  • Hamouda H, Kaup M, Ullah M, Berger M, Sandig V, Tauber R, Blanchard V (2014) Rapid and specific analysis of cell surface N-glycosylation of living cells. J Prot Res, 13(12):6144-51.
  • Weiz S, Kamalakumar A, Biskup K and Blanchard V (2015) Enhanced detection of in-gel released N-glycans by MALDI-TOF-MS. Proteomics, 15(9):1503-7.
  • Rosenlöcher J, Weilandt C, Sandig G, Reinke S, Blanchard V and Hinderlich S (2015) The human rhabdomyosarcoma cell line TE671 – Towards an innovative production platform for glycosylated biopharmaceuticals, Protein Expression and Purification, 115:83-94.
  • Rosenlöcher J, Böhrsch V, Sacharjat M, Blanchard V, Sandig V and Hinderlich S (2015) Applying acylated fucose analogues to metabolic glycoengineering. Bioengineering, 2(4), 213-234.
  • Rosenlöcher J, Sandig G, Kannicht C, Blanchard V, Reinke S and Hinderlich S (2016) Recombinant glycoproteins: The impact of cell lines and culture conditions on the generation of protein species, J Proteomics, 134:85-92.
  • Weiz S, Wieczorek M, Schwedler C, Kaup M, Braicu EI, Sehouli J, Tauber R and Blanchard V (2016) Acute-phase glycoprotein N-glycome of ovarian cancer patients analyzed by CE-LIF, Electrophoresis, 37:1461-7.
  • Braig F, Kriegs M, Voigtlaender M, Habel B, Grob T, Biskup K, Blanchard V, Sack M, Thalhammer A, Ben Batalla I, Braren I, Laban S, Danielczyk A, Goletz S, Jakubowicz E, Märkl B, Trepel M, Knecht R, Riecken K, Fehse B, Loges S, Bokemeyer C and Binder M (2017) Cetuximab resistance in head and neck cancer is mediated by EGFR-K521 polymorphism. Cancer Res., 77(5)1188-1199.
  • Biskup K, Braicu EI, Sehouli J, Tauber R and Blanchard V (2017) The ascites N-glycome of epithelial ovarian cancer patients. J Proteomics, 157, 33-39.
  • Montacir H, Freyer N, Knöspel F, Urbaniak T, Dedova T, Berger M, Damm G, Tauber R, Zeilinger K and Blanchard V (2017) The N-glycome of embryonic stem cells and thereof differentiated hepatic cells. ChemBioChem, 8(13):1234-1241.
  • Sandig G, Von Horsten HH, Radke L, Blanchard V, Frohme M, Giese C, Sandig V and Hinderlich S (2017) Engineering of CHO cells for the production of recombinant
  • glycoprotein vaccines with xylosylated N-glycans. Bioengineering, 4(2). pii: E38.
  • Sun T, Ruscito I, Dimitrova D, Chekerov R, Kulbe H, Baron U, Blanchard V, Panici PB, Darb-Esfahani S, Sehouli J, Olek S and Braicu EI (2017) Genetic versus epigenetic BRCA1 silencing pathways: clinical effects in primary ovarian cancer patients: a study of the Tumor Bank Ovarian Cancer Consortium. Int J Gynecol Cancer, 27(8):1658-1665.
  • Radke L, Sandig G, Lubitz A, Schließer U, Von Horsten H, Blanchard V, Keil K, Sandig V, Giese C, Hummel M, Hinderlich S and Frohme M (2017) In-vitro evaluation of glycoengineered RSV-F in the Human artifical Lymph Node Reactor. Bioengineering, 4(3). pii: E70.
  • Zhang, R, Blanchard V, Vera M and Sand W (2017) EPS Characterization of a Cell Wall-Lacking Archaeon Ferroplasma acidiphilum. Solid State Phenomena, 262:434-8.
  • Epp A, Hobusch J, Bartsch YC, Petry J, Lilienthal GM, Koeleman CM, Eschweiler S, Möbs C, Hall A, Morris SC, Petzold D, Engellenner C, Bitterling J, Rahmöller J, Leliavski A, Thurmann R, Collin M, Moremen KW, Strait RT, Blanchard V, Petersen A, Gemoll T, Habermann JK, Petersen F, Nandy A, Kahlert, PhD H, Hertl M, Wuhrer M, Pfützner W, Jappe U, Finkelman FD, and Ehlers M (2018) Sialylation of IgG antibodies inhibits IgG-mediated allergic reactions. J Allergy Clin Immunol, 141(1):399-402.
  • Schwedler C, Häupl T, Kalus U, Blanchard V, Burmester GR, Poddubnyy D, Hoppe B (2018) Hypogalactosylation of immunoglobulin G in rheumatoid arthritis: Correlation with HLA-DRB1 shared epitope, anti-citrullinated protein antibodies, rheumatoid factor and inflammatory activity. Arthritis Res Ther, 20(1):44.
  • Brockmann T, Blanchard V, Heretsch P, Brockmann C and Bertelmann E. Photochemical degradation of trypan blue. Plos One, 13(4):e0195849.
  • Bartsch Y, Rahmöller J, Mertes MMM, Eiglmeier S, Lorenz F, Stoehr A, Braumann D, Lorenz L, Winkler A, Lilienthal GM, Petry J, Hobusch J, Steinhaus M, Hess C, Holecska V, Schoen CT, Oefner C, Leliavski A, Blanchard V and Ehlers M (2018) Sialylated autoantigen-reactive IgG antibodies attenuate disease development in autoimmune mouse models of lupus nephritis and rheumatoid arthritis. Front Immunol, 9:1183.
  • Dědová T, Grunow D, Kappert K, Flach D, Tauber R and Blanchard V (2018) The effect of blood sampling and preanalytical processing on human N-glycome. Plos One, 13(7):e0200507.
  • Nietzold C, Dietrich PM, Holzweber M, Lippitza A, Kamalakumar A, Blanchard V, Ivanov-Pankov S, Weigel W, Pannea U, Unger WES. Surface chemical characterization of model glycan surfaces and shelf life studies of glycan microarrays using XPS, NEXAFS spectroscopy, ToF-SIMS and fluorescence scanning (2018) Appl Surf Sci, 459:860-873.
  • Zhang R, Neu TR, Blanchard V, Vera M, Sand W. Biofilm dynamics and EPS production of a thermoacidophilic bioleaching archaeon (2019) New Biotechnology, 51:21-30.
  • Dědová T, Braicu EI, Sehouli J and Blanchard V. Sialic acid linkage analysis refines the diagnosis of ovarian cancer (2019) Front Oncol, 9:261.
  • Zhang R, Neu TR, Li, Q Blanchard V, Zhang, Y and Sand W. Insight into interactions of thermoacidophilic archaea with elemental sulfur: biofilm dynamics and EPS analysis (2019) Front Microbiol, 10:896.
  • Wieczorek M, Braicu EI, Oliveira-Ferrer L, Sehouli J and Blanchard V. Immunoglobulin G subclass-specific glycosylation changes in primary epithelial ovarian cancer (2020) Front Immunol 11:654.
  • Bartsch YC, Eschweiler S, Leliavski A, Lunding HB, Wagt S, Petry J, Lilienthal GM, Rahmöller J, de Haan N, Hölscher A, Erapaneedi R, Giannou AD, Aly L, Sato R, de Neef LA, Winkler A, Braumann D, Hobusch J, Kuhnigk K, Krémer V, Steinhaus M, Blanchard V, Gemoll T, Habermann JK, Collin M, Salinas G, Manz RA, Fukuyama H, Korn T, Waisman A, Yogev N, Huber S, Rabe B, Rose-John S, Busch H, Berberich-Siebelt F, Hölscher C, Wuhrer M, and Ehlers M. IgG Fc sialylation is regulated during the germinal center reaction upon immunization with different adjuvants (2020) J. Allergy Clin. Immunol., in press.
  • Biskup K*, Blanchard V*, Castillo-Binder P, Alexander H, Engeland K and Schug S. N- And O-glycosylation patterns and functional testing of CGB7 versus CGB3/5/8 variants of the human chorionic gonadotropin (hCG) beta subunit (2020). Glycoconj. J., in press.
  • * authors contributed equally.

Reviews und Monografien

  • Gohlke M, and Blanchard V (2008) Separation of N-glycans by HPLC, In Methods in Molecular Biology, C. Kannicht, ed. 446, 239-54.
  • Berger M, Kaup M, and Blanchard V (2012) Protein glycosylation and its impact on Biotechnology. Adv Biochem Eng Biotechnol, 127:165-85.
  • Wedepohl S, Beceren-Braun F, Riese S, Buscher K, Enders S, Bernhard G, Kilian K, Blanchard V, Dernedde J and Tauber R (2012) L-Selectin - A dynamic regulator of leukocyte migration. Eur J Cell Biol, 91(4):257-64.
  • Schwedler C and Blanchard V (2019) Measurement of neutral and sialylated IgG N-glycome at Asn-297 by CE-LIF to assess hypogalactosylation in rheumatoid arthritis. In Methods in Molecular Biology, T. Phillips, ed., 1972:77-93.
  • Gohlke M, and Blanchard V (2019) Chromatographic Profiling of N-Glycans, In Methods in Molecular Biology, C. Kannicht, ed., 934:65-81.
  • Grunow D and Blanchard V (2019) Enzymatic Release of Glycoprotein N-Glycans and Fluorescent Labeling. In Methods in Molecular Biology, C. Kannicht, ed., 1934:43-49.
  • Blois SM, Dveksler G, Vasta GR, Freitag N, Blanchard V, Barrientos G. Pregnancy Galectinology: Insights into a Complex Network of Glycan Binding Proteins (2019) Front Immunol, 10:1166.
  • * authors contributed equally.